rabbit polyclonal anti cleaved dcp1 cell signaling technologies ab 2721060  (Cell Signaling Technology Inc)

Journal: PLOS Biology

Article Title: Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras

doi: 10.1371/journal.pbio.3003068

Figure Lengend Snippet: (A) Confocal images of EADs and tumors of indicated genotypes at 96-h AED labeled for Dcp1 illustrating the enrichment of cell death after Dorsal overexpression in control EADs as quantified in (B) and demonstrating the loss of cell death after inhibition of apoptosis through overexpression of Ras V12 or p35. For simplicity, all genotypes with overexpression of p35 are only shown with a nuclear marker (gray); a quantification of the Dcp1-positive area can, however, be found in S4F Fig. The white outline highlights the GFP-marked region. (B) Quantification of the Dcp1 positive area relative to whole disc size at 96-h AED. Mean area and standard deviation (error bar) are shown (*** p < 0.001, One-way ANOVA with post-hoc Tukey HSD). (C) qRT-PCR analysis of rpr mRNA levels in EADs expressing indicated UAS-transgenes under the control of an eye-specific Gal4 ( eyFlp; act>CD2>Gal4 ) illustrating the increase in pro-apoptotic gene expression after overexpression of Dorsal in control EADs. Data is shown as fold changes relative to the control (lacZ). n = 3 or n = 4 biologically independent samples were analyzed. Median fold changes and interquartile ranges (error bars) are shown. The dashed horizontal line illustrates the reference fold change of 1. (D) Quantification of tissue size of indicated genotypes at 96-h AED to illustrate changes to the effects of Dorsal on tissue growth after inhibition of apoptosis through co-expression of p35. Mean area and standard deviation (error bar) are shown (*** p < 0.001, One-way ANOVA with post-hoc Tukey HSD). (E) Graphical model illustrating the transition of the function of NF-κB/ Dorsal from growth-suppressive during development to growth-promoting during Ras V12 -mediated oncogenesis. The transition is mediated by the combined effects of Dorsal-induced changes in cell death, cell survival, proliferation and differentiation. In all confocal images, DAPI (gray) is used to visualize nuclei and GFP (green) labels cells co-expressing indicated UAS-transgenes. The underlying data sets can be found in . Scale bars represent 100 µm. EAD, eye-antennal disc; AED, after egg deposition; Dl, Dorsal; Dcp1, cleaved Drosophila caspase 1; rpr, reaper; NF-κB, nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells.

Article Snippet: rabbit polyclonal anti-cleaved Dcp1 , Cell Signaling Technologies , AB_2721060 , 1:200.

Techniques: Labeling, Over Expression, Control, Inhibition, Marker, Standard Deviation, Quantitative RT-PCR, Expressing, Gene Expression

Journal: PLOS Biology

Article Title: Activation of the Drosophila innate immune system accelerates growth in cooperation with oncogenic Ras

doi: 10.1371/journal.pbio.3003068

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: rabbit polyclonal anti-cleaved Dcp1 , Cell Signaling Technologies , AB_2721060 , 1:200.

Techniques:

Journal: eLife

Article Title: A neurotrophin functioning with a Toll regulates structural plasticity in a dopaminergic circuit

doi: 10.7554/eLife.102222

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-DCP-1 , Cell Signalling , Cat# 8578S , 1:250.

Techniques: CRISPR, Reverse Transcription, Plasmid Preparation, Software

Journal: The EMBO Journal

Article Title: Nuclear receptor E75/NR1D2 promotes tumor malignant transformation by integrating Hippo and Notch pathways

doi: 10.1038/s44318-024-00290-3

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit polyclonal anti-Cleaved Drosophila Dcp-1 , Cell Signaling Technology , Cat# 9578; RRID: AB_2721060.

Techniques: Protease Inhibitor, In Situ, Transfection, Magnetic Beads, Plasmid Preparation, cDNA Synthesis, Cell Viability Assay, Concentration Assay, Software